Youming Xie, Ph.D.
421 E. Canfield Street, Suite 1146
Detroit, MI 48201
- We use both Saccharomyces cerevisiae and mammalian systems to define the pathways that control the degradation of key regulatory proteins.
- In addition, we develop novel approaches to target the proteasome for cancer therapy.
The focus of our research is to understand the molecular mechanisms underlying protein degradation mediated by the ubiquitin-proteasome system (UPS). UPS is the primary intracellular machine responsible for elimination of abnormal proteins and selective destruction of regulatory proteins involved in a wide range of cellular processes including cell cycle control, DNA transcription, replication and repair, and stress response. Dysregulation of UPS is implicated in various human diseases including cancer. Moreover, UPS has now become an important target for anti-cancer therapy. We use both Saccharomyces cerevisiae and mammalian systems to define the pathways that control the degradation of several key regulatory proteins. In addition, we are studying the differential responsees of normal cells and cancer cells to inhibitors of UPS.
Ju D, Xie Y. Dyclonine enhances the cytotoxic effect of proteasome inhibitor bortezomib in multiple myeloma cells. Mol Med Rep. 2014;10:2609-12.
Wang Y, Ha SW, Zhang T, Kho DH, Raz A, Xie Y. Polyubiquitylation of AMF requires cooperation between the gp78 and TRIM25 ubiquitin ligases. Oncotarget. 2014;5:2044-51.
Lee JS, Zheng Z, Mendez R, Ha SW, Xie Y, Zhang K. Pharmacologic ER stress induces non-alcoholic steatohepatitis in an animal model. Toxicol Lett. 2012;211:29-38.
Ha SW, Ju D, Xie Y. The N-terminal domain of Rpn4 serves as a portable ubiquitin-independent degron and is recognized by specific 19S RP subunits. Biochem Biophys Res Commun. 2012;419:226-31.
Xu H, Fu J, Ha SW, Ju D, Zheng J, Li L, Xie Y. The CCAAT box-binding transcription factor NF-Y regulates basal expression of human proteasome genes. Biochim Biophys Acta. 2012;1823:818-25.
Education and Training:
PhD in Cell Biology (1996): University of Texas, MD Anderson Cancer Center, Houston, Texas
Post-Doc in Biochemistry (1996-2002): California Institute of Technology, Pasadena, California
Cancer Biology Courses Taught:
CB7210 Fundamentals of Cancer Biology (Course Co-Director)
CB7220 Molecular Biology of Cancer Development
CB7300 Special Topics F31 Grant Writing Course