Address4100 John R St HWCRC Room 624 Detroit, MI 48201
4100 John R St
HWCRC Room 624
Detroit, MI 48201
Ion John Campeanu (MD/PhD)
Laboratory Web Site
- Stem cell and Cancer Epigenetics
- Transcriptional Networks
- Reprogramming and Transdifferentiation
- Genome Stability
One of the research goals of Dr. Benjamin Kidder's lab is to understand how stem cells pattern the epigenetic landscape in a way that facilitates distinct expression programs throughout development. Humans develop from a single fertilized egg into a complex organism with many cell types, each with its own distinct gene expression and epigenetic profile. These complex cellular states, when perturbed, can lead to diseases such as cancer. Our lab uses experimental, genomics, and computational biology tools to study the epigenetic regulation of stem cell and cancer function, genome stability, and reprogramming/transdifferentiation. We are particularly interested in understanding how epigenetic landscapes are regulated in cancer vs. normal cells, the role for heterochromatin in regulating genome stability, and how histone modifying enzymes contribute to the diverse cellular repertoire that exists in mammals.
KDM5B is a master regulator of the H3K4-methylome in stem cells, development and cancer. Xhabija B, Kidder BL. Semin Cancer Biol. 2019;57:79-85.
Contribution of H3K4 demethylase KDM5B to nucleosome organization in embryonic stem cells revealed by micrococcal nuclease sequencing. Kurup JT, Campeanu IJ, Kidder BL. Epigenetics Chromatin. 2019;12:20.
Kurup JT, Kidder BL. Identification of H4K20me3 and H3K4me3-associated RNAs using CARIP-Seq expands the transcriptional and epigenetic networks of embryonic stem cells. J Biol Chem. 2018 Aug 16. pii: jbc.RA118.004974. [Epub ahead of print].
He R, Kidder BL. Culture of haploid blastocysts in FGF4 favors the derivation of epiblast stem cells with a primed epigenetic and transcriptional landscape. Sci Rep. 2018;8:10775.
Xu J, Kidder BL. H4K20me3 co-localizes with activating histone modifications at transcriptionally dynamic regions in embryonic stem cells. BMC Genomics. 2018;19:514.
Kidder BL. CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells. J Vis Exp. 2018;29889205.
Xu J, Kidder BL. KDM5B decommissions the H3K4 methylation landscape of self-renewal genes during trophoblast stem cell differentiation. Biol Open. 2018;7:bio031245.
Vizcardo R, Klemen ND, Islam SMR, Gurusamy D, Tamaoki N, Yamada D, Koseki H, Kidder BL, Yu Z, Jia L, Henning AN, Good ML, Bosch-Marce M, Maeda T, Liu C, Abdullaev Z, Pack S, Palmer DC, Stroncek DF, Ito F, Flomerfelt FA, Kruhlak MJ, Restifo NP. Generation of Tumor Antigen-Specific iPSC-Derived Thymic Emigrants Using a 3D Thymic Culture System. Cell Rep. 2018;22:3175-90.
He R, Xhabija B, Al-Qanber B, Kidder BL. OCT4 supports extended LIF-independent self-renewal and maintenance of transcriptional and epigenetic networks in embryonic stem cells. Sci Rep. 2017;7:16360.
Kidder BL*, He R, Wangsa D, Padilla-Nash HM, Bernardo MM, Sheng S, Ried T, Zhao K*. SMYD5 controls heterochromatin and chromosome integrity during embryonic stem cell differentiation. Cancer Res. 2017;77:6729-45.
He R, Kidder BL. H3K4 demethylase KDM5B regulates global dynamics of transcription elongation and alternative splicing in embryonic stem cells. Nucleic Acids Research 2017;45:6427-41
Kidder BL*, Hu G, Cui K, Zhao K*. SMYD5 regulates H4K20me3-marked heterochromatin to safeguard ES cell self-renewal and prevent spurious differentiation. Epigenetics And Chromatin 2017;10:8.
Rosati R, Patki M, Chari V1, Dakshnamurthy S, McFall T, Saxton J, Kidder BL, Shaw PE, Ratnam M. The Amino-terminal Domain of the Androgen Receptor Co-opts Extracellular Signal-regulated Kinase (ERK) Docking Sites in ELK1 Protein to Induce Sustained Gene Activation That Supports Prostate Cancer Cell Growth. J Biol Chem 2016;291:25983-98.
B.A. in Chemistry and Biology (2001): Saint Olaf College, Northfield, MN
Ph.D. in Molecular, Cellular, Developmental Biology and Genetics (2007): University of Minnesota, Minneapolis, MN
Postdoctoral Fellow: EMD/Merck Serono Research Institute, Rockland, MA
Principal Investigator: EMD/Merck Serono Research Institute, Rockland, MA
Research Fellow in Epigenome Biology: National Heart, Lung and Blood Institute (NHLBI, NIH), Bethesda, MD
CB7210 Fundamentals of Cancer Biology
CB7700 Recent Developments in Cancer Biology